Kiwamu Tanaka
Dept. of Plant Pathology
Washington State Universityt
Pullman
USA
Samodia Jayasinghe
Dept. of Plant pathology
Washington State University
Pullman
USA
e-mail:
e-mail:
Powdery scab situation
Research
Sporosorus formation within root galls was investigated, using scanning electron microscopy and light microscopy. Starch grains accumulated during early stages root gall development, and these gradually diminished as the galls matured
to brown colour containing fully formed sporosori. Light microscopy of ultramicrotome sections showed presence of high-amylopectin starches enveloped by plasmodia as precursors to sporosori, indicating involvement of starch in sporosorus formation.
Potato hairy roots were used to study gene expression responses to Sss, and to validate gene involvement using gene editing. Following Sss inoculations, differential expression of plant defense-related genes was observed in the hairy roots. Based on defense marker
gene expression, a pivotal role was indicated of salicylic acid (SA)-mediated defense against Sss. This was further investigated by assessing the impact of SA-mediated defense on Sss infection. Transcriptomics using RNA-seq on hairy root samples from different
cultivars after Sss infection has given insights into involvement of SA-mediated defense and other potential mechanisms for preventing Sss infections.
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Last update: January, 2025